Publikasi Dosen

Characterization of lytic phage Staphylococcus aureusfrom dairy farm cows in Indonesia

Abstract :
Staphylococcus aureus is one of pathogenic bacteria causing multitude of diseases. This bacterium was resistant to ampicillin, tetracycline and chloramphenicol. Therefore, to
reduce potential infection of antibiotic resistant S. aureus, alternative solutions such as application of lytic phage were needed. The aims of this study were to isolate and characterize
phage that can lyse S. aureus cells. Phages were isolated from waste water from local dairy farm. Phage isolates were determined by their ability to form plaques, specificity, morphology,
and effectiveness of bacteriolysis. In this study, 3 phages (FSb, FSs, and FSk) were isolated to replicate S. aureus isolates as a potential way to control these infections. Phage was specific to
S. aureus as its host. Transmission electron microscope observation showed that the FSb and FSk can be classified as a member of Myoviridae family, while FSk was the member of
Siphoviridae family. FSb had an icosahedral head in 85.71 nm diameter, long tail of 126.67 nm, and the diameter of the tail about 20.00 nm width; FSs had an icosahedral head in 53.33 nm
diameter, long tail of 106.67 nm, and the diameter of the tail about 13.33 nm width; FSk had an icosahedral head in 66.67 nm diameter, long tail of 120.00 nm, and the diameter of the tail
about 16.67 nm width. All lytic phage reduced the population of S. aureus effectively. Lytic phages found in this study can be used as an alternative therapy agent to S. aureus infected.
Keywords: Characterization, lytic phage, Staphylococcus aureus.

Ketersediaan

Informasi Detail

Judul Seri

-

No. Panggil

001

Penerbit

CODEN (USA) : IJPRIF., 2016

Deskripsi Fisik

-

Bahasa

Indonesia

ISBN/ISSN

-

Klasifikasi

001

Tipe Isi

-

Tipe Media

-

Tipe Pembawa

-

Edisi

2021

Subjek

Characterization, lytic phage, Staphylococcus au

Info Detail Spesifik

Author Priyanto Dwi Nugroho1; Sri Budiarti2; Iman Rusmana2 -- 1.Department of Pharmaceutical and Food Analyst, Ministry of Health, Health Polytechnic of Jakarta 2, Jakarta, Indonesia; 2.Department of Biology, Faculty of Mathematics and Natural Sciences, Bogor Agricultural ; University, Dramaga Campus, Bogor 16680, Indonesia; Jurnal : International Journal of PharmTech Research CODEN (USA): IJPRIF, ISSN: 0974-4304, ISSN(Online): 2455-9563 ; Vol.9, No.11, pp 10-18, 2016; DOI: https://doi.org/10.36525/sanitas.2017.9;

Pernyataan Tanggungjawab

-

Versi lain/terkait

Lampiran Berkas

Komentar